
HCC-827
ACC 566
Cell line:
HCC-827
DSMZ no.:
ACC 566
Species:
human (Homo sapiens)
Cell type:
non-small cell lung carcinoma
Origin:
established from the lung of a 39-year-old woman with non-small cell lung cancer of the adenocarcinoma type; extensively characterized cell line (see ref 40398 and Cellosaurus:Expasy; listed in the CCLE (see ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:2 to 1:3 about twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 5-6 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 6% polyploidy - 62(54-66)<3n>XXX/XXXX, -1, +7, +7, -9, -10, -15, -17, -18, -21, -22, +3 mar, +0-4 dmin, add(X)(p11), dup(2)(p1?p2?), add(3)(p11), add(6)(p21), add(7)(p12), add(7)(p22), add(7)(q21.2;HSR), add(21)(p11), der(21;22)(q10;q10), add(22)(p11) - 2 large submetacentric HSR markers present
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list