HELA
ACC 57
Cell line:
HELA
DSMZ no.:
ACC 57
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma
Origin:
established from the epitheloid cervix carcinoma of a 31-year-old woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid, continuously cultured human cell line
Biosafety level:
1
Risk assessment:
The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% MEM (with Earle's salts) + 5-10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids (cells also grow well in 90-95% RPMI 1640 + 5-10% h.i. FBS)
Subculture:
seed out at ca. 0.5-2 x 106 cells in a T75 flask; split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid/hypotetraploid karyotype with 15% polyploidy - 82(77-84)<4n>XXX, -X, -2, -3, -4, +5, +5, +5, +5, -7, -8, -11, -13, -14, -15, -16, -17, -18, -19, -21, -22, +mar - der(1)t(1;3)(p11;q11), add(2)(q37), del(3)(q13), der(3)t(3;5)(q11;q11), i(5p)x4, del(6)(q15), der(9)t(1;9)(p11;p11), add(12)(q23), i(15q), der(19)t(13;19)(q22;p13)x1-2, add(21)(p11), del(?22)(q12)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list