SU-DHL-8
ACC 573
Cell line:
SU-DHL-8
DSMZ no.:
ACC 573
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the pleural effusion of a 59-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell lymphoma, noncleaved cell type and later as diffuse histiocytic lymphoma; assigned to GCB-like lymphoma subtype (germinal center B-cell); carries the t(8;22) translocation affecting c-myc
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml; maximal density at 3-4 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
cell harvest of ca. 3-4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD10-, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80+, CD138-, HLA-DR+, sIgG+, sIgM-, sIgkappa+; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 3% polyploidy - 51(47-53)<2n>X, -Y, +7, +13, +20, +20, t(8;22)(q24;q11), del(20)(q13), der(22)t(1;22)(q11;p11)del(1)(q?32) - carries t(8;22) effecting IGL-MYC rearrangement - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list