SU-DHL-16
ACC 577
Cell line:
SU-DHL-16
DSMZ no.:
ACC 577
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the pleural effusion of a man with B-cell non-Hodgkin lymphoma (B-NHL)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml in a 24-well-plate, after thawing viability may be less than 70% but the cells recover during the following 3-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 3% polyploidy - 47(44-49)<2n>XXY, +10, -13, +15, t(1;5)(q31;q21), add(2)(q21-22), der(2)t(2;2)(q3?7;q21-22)t(2;10)(p25;q24), der(10)t(2;10)(p25;q24)x2, del(12)(p12)dup(12)(q11q22), der(14)t(13;14)(q11;p11)t(14;18)(q32;q21), add(15)(q23)x2, i(17q), der(18)t(14;18)(q32;q21) - carries t(14;18) effecting BCL2-IGH rearrangement
Molec. Genetics:
expression of fusion gene BCL2-IGH confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list