HCT-116
ACC 581
Cell line:
HCT-116
DSMZ no.:
ACC 581
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the primary colon carcinoma of an adult man; cells were described in the literature to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% McCoy's 5A + 10% h.i. FBS + 2 mM L-glutamine (alternatively DMEM with high glucose)
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:3 to 1:6 about 2-3 x per week using trypsin/EDTA; in confluent cultures with high densities, cells may detach spontaneously
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-40 hours
Harvest:
cell harvest of ca. 10-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes
Viruses:
PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list