Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peripheral blood of a 57-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphocytic, small cell type progressing to large non-convoluted cell type) in the refractory terminal stage in 1977; assigned to ABC-like lymphoma subtype (activated B-cell); cell line also known as Riva. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
large round cells growing in suspension;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
maximum density at about 3-4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture and PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 4% polyploidy - 48-51<2n>XX, +7, +18, +19, +mar, t(1;22;16)(p35;p12;q12.2), del(3)(q11-12), trp(3)(q11-12q28-29), t(4;8)(q22;q24), del(6)(q13), der(15)t(13;15)(q11;p1?1), der(18)amp(18)(q21)dup(18)(q21q23), der(19)t(6;19)(p21;p13)add(19(q13.3) - carries t(4;8) and der(18) effecting MYC rearrangement and amplification of BCL2, respectively - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
