
JIYOYE
ACC 590
Cell line:
JIYOYE
DSMZ no.:
ACC 590
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
cell line JIYOYE (also known as JIJOYE, P-2003 or P-3) was established from a 7-year-boy with Burkitt lymphoma in 1965
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5-0.8 x 106 cells/ml in 24-well-plates in 20% FBS; after recovery, viability may be low! maintain at ca. 0.3-1.0 x 106 cells/ml; maximum density at ca. 2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-60 hours
Harvest:
maximum density at about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +,
CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype with 13% polyploidy - 42-48<2n>XY, der(7)t(1;7)(q32;q32), t(8;14)(q24;q32), i(9p), der(14)t(9;14)(q11;q32), +0-2mar; carries t(8;14) rearranging IGH with MYC (far upstream breakpoint)
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list