human (Homo sapiens)
established from a pleural biopsy of a 62-year-old man with malignant mesothelioma
Permissions and restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
80% Dulbecco's MEM or NCTC-109 + 20% h.i. FBS
split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109)
ca. 50-60 hours
cell harvest of ca. 6-8 x 106 cells/175 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in microbiological culture, PCR assays
To inquire about expression of EpCAM and intermediate filaments, contact firstname.lastname@example.org.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human near-diploid karyotype with 16% polyploidy; 46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
|Frozen culture||450,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||560,- €|
|DNA isolated from cell line (5 µg)||140,- €|
see price list