DM-3
ACC 595
Cell line:
DM-3
DSMZ no.:
ACC 595
Species:
human (Homo sapiens)
Cell type:
sarcomatoid mesothelioma
Origin:
established from a pleural biopsy of a 62-year-old man with malignant mesothelioma
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% Dulbecco's MEM or NCTC-109 + 20% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109)
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 6-8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 16% polyploidy;
46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list