BEN-MEN-1
ACC 599
Cell line:
BEN-MEN-1
DSMZ no.:
ACC 599
Species:
human (Homo sapiens)
Cell type:
benign meningioma
Origin:
established in 2003 from the meningothelial meningioma (grade I, WHO, #1238-03) attached to the parietal falx of a 68-year-old woman after surgical tumor resection; cells were immortalized by retroviral transduction with human telomerase reverse transcriptase (hTERT)
Reference(s):
Biosafety level:
1, GMO-S1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS
Subculture:
seed out at ca. 0.7-2.0 x 106 cells/175 cm2; optimal split ratio 1:2 to 1:5 every 2-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 12% polyploidy; 44(43-46)<2n>XX, -22; frequent random chromosome loss; monosomy 22 is recurrent in grade I benign meningiomas; matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list