Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described in the literature to be EBV-negative but EBV infectible, to carry the (8;14) translocation and TP53 mutations. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Morphology:
round cells growing singly or in clumps in suspension;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; medium must be changed frequently to prevent acidification and the specified cell density should be complied
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype; 45(44-46)<2n>X, -Y, del(2)(p16p23), t(8;14)(q24;q32.2), add(14)(p11), der(16)t(7;16)(q11.2;p13.2), der(17)t(13;17)(q33;p11.3)t(13;3)(q13;q?25), del(20)(q12); carries t(8;14) effecting IGH-MYC rearrangement
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
