established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described in the literature to be EBV-negative but EBV infectible, to carry the (8;14) translocation and TP53 mutations. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq)
round cells growing singly or in clumps in suspension; image; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; medium must be changed frequently to prevent acidification and the specified cell density should be complied
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays
