MOLP-2

human (Homo sapiens)

multiple myeloma

established from the peripheral blood of a 55-year-old man with IgD lambda type multiple myeloma in 1991

1

A

round to polymorph cells growing singly or in small clumps, ca. 1% giant cells; image; image; image

80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine

seed out at ca. 0.5 x 10⁶ cells/ml in 24-well plates; split culture 1:2 every 4-6 days; maintain at ca. 0.2-0.8 x 10⁶ cells/ml; after thawing cells may need one week before they start to grow (and they grow very slowly at all)

at 37 °C with 5% CO₂

ca. 4-7 days

maximum density at ca. 1 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD33 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 +, sm/cyIgG -, sm/cyIgM -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human highly-rearranged, flat-moded, near-triploid karyotype; 66-74<3n>XX, -Y, +2, -4, +6, +7, +11, -15, +17, -18, -18, +19, +20, -22, -22, -22, +3-5mar, der(X;1)(q10;p10), add(2)(q21), der(2)t(2;15)(q37;q14)x2, der(2)t(1;2)(q2?;p1?6), add(5)(q21), del(5)(q12), add(7)(p21)x2, add(11)(p13), der(16)t(1;16)( p21;q11), add(16)(p11)t(14;16)(q32;q22)t(14;22)(q32;q11.1), der(17)t(17;22)(q25;q11)x2, der(21)t(4;21)(q21.2;p11)inv(4), der(21)t(4;21)(q21.2;p11)inv(4)(?p16?q21)t(4;14)(q?35;q32); sdl with der(6)t(6;18)(q21;q12)x2-3; carries multiple complex rearrangements, including cryptic t(4;14)(p16;q32) rearranging NSD2-FGFR3 (WHSC1-FGFR3; MMSET-FGFR3 with IGH

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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