HAL-01
ACC 610
Cell line:
HAL-01
DSMZ no.:
ACC 610
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 1-2 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week; adherent cells can be detached by tapping the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD10+, CD13-, CD19+, CD20(+), CD34-, CD37+, CD38+, CD80-, CD138+, HLA-DR+, sIgkappa-, cIgkappa-, sIglambda-, cIgkappa-, sIgG-, cIgG-, sIgM-, cIgM-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 4% polyploidy;
46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype
Molec. Genetics:
expression of fusion gene TCF3-HLF (E2A-HLF) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list