RL
ACC 613
Cell line:
RL
DSMZ no.:
ACC 613
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1983 from the ascites of a 52-year-old man with B-NHL (diffuse, undifferentiated, small non-cleaved large cell)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.7-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 5-8 days
Harvest:
maximum density at ca. 1.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 16% polyploidy; 49(45-51)<2n>XY, +7, +17, +21, der(3)inv(3)(p24q24-q25)t(3;13)(q24-q25;q32), add(5)(p11), der(13)t(3;13)(q24-q25;q32), t(14;18)(q32;q21), der(17)dup(17)(p12p13)t(17;20)(q11.2;q11.2), r(17), add(19)(q12), der(19)t(19;20)(p13;p11), der(20)t(17;20)(q11.2;q11.2)x1-2; carries t(14;18) effecting rearrangement of BCL2 with IGH (IGH); resembles published karyotype
Molec. Genetics:
expression of fusion gene IGH-BCL2 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list