SCC-25
ACC 617
Cell line:
SCC-25
DSMZ no.:
ACC 617
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from the biopsy of a squamous cell carcinoma of the tongue of a 70-year-old man; described to carry a TP53 mutation
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% mixture of Ham's F12 + DMEM (at 1:1) + 20% h.i. FBS, 2 mM L-glutamine, 1 mM sodium pyruvate
Subculture:
seed out at ca. 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA (wash twice with PBS before trypsination)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged hypertriploid karyotype with 4% polyploidy; 72-82<3n>XX, -Y, +4, -7, +9, +9, -13, -14, +15, -18, -19, -21, +10, -12 mar; add(1)(p31)x2, add(3)(?p11), add(4)(q11), i(5p), i(5q), der(8)t(1;8)(q25;p11)x1-2, del(9)(p11), i(9q), add(11)(q25), i(13q), add(14)(p11), add(15)(p11)x3-4, add(21)(q22)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list