MEL-HO
ACC 62
Cell line:
MEL-HO
DSMZ no.:
ACC 62
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the primary tumor of a woman with melanoma in 1976
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
95% RPMI 1640 + 5% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:5 to 1:10 using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
about 10-12 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 1.5% polyploidy - 74(72-76)<3n>XX, -X, +3, +7, +7, -8, -10, -11, +15, -16, +17, +18, +20, +21, +mar - del(1)(q42), del(3)(p21), i(7q)x2, del(9)(p13), der(15)t(15;?)(q21;?), i(17q), del(X)(q11)
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list