
SK-N-BE(2)
ACC 632
Cell line:
SK-N-BE(2)
DSMZ no.:
ACC 632
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established in 1972 from the bone marrow biopsy of a 22-month-old boy with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy; described to carry mutations in the TP53 gene
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% mixture of Ham's F12 + MEM (with Earle's salts)(at 1:1) + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:6 - 1:10 every week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
maximum density at ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 12% polyploidy
43<2n>(42-49)X, -Y, +6, -9, -17, -18, der(1)t(1;2)(p13;p15), der(2)i(2)(p10)del(2)(p?13p24), der(3)t(3;17)(p21;q21), add(4)(q25), der(4)t(1;4)(q21;p15), add(6)(q25), add(8)(p12), der(10)add(10)(p13)add(10)(q21)(hsr), add(11)(p13), add(11)(q11), add(14)(q31), add(19)(q12); carries large hsr marker with genomic amplification of NMYC; resembles published karyotypes
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list