Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq).
Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
small round cells growing singly and in clusters in suspension;
image;
image;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:3 twice a week, maintain at 1-1.5 x 106 cells/ml; could be of advantage to start culture in a 12- or 24-well plate
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3-, CD10+, CD13-, CD19+, CD20+, CD37+, CD38+, cyCD79a+, CD80+, CD138+, HLA-DR+, sIgG-,cIgG-, sIgM+, cIgM+ sIgkappa+, cIgkappa+, sIglambda-, cIglambda-;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human polyclonal hypodiploid karyotype with 20% polyploidy; 45(43-46)<2n>, XX, add(X)(q22), add(1)(q24), der(3)ins(3;18)(q27;q2?q2?)hsr(18)(q21), add(5)(q32), der(6)t(6;18)(p24;?q23)del(6)(q13), der(10)t(10;14)(q24;q23), der(11)t(1;11)(q25;q2?), der(14)t(3;14)(q27;p11), der(18)t(1;18)(q21;q21), der(18)t(3;18)(q2?;q27), add(19)(p13), del(19)(q13); sdl with del(2)(q11), t(4;15)(q22;q14), add(7)(q21), del(13)(?q21), der(13)add(13)(p11)add(13)(q32); extensive genomic amplification of BCL2 region; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
