DT-40
ACC 636
Cell line:
DT-40
DSMZ no.:
ACC 636
Species:
chicken (Gallus gallus)
Cell type:
B cell line
Origin:
established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described in the literature to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing in clumps of 10 to 100 cells in suspension
Medium:
80% Advanced RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine + 4.5 g/L glucose + 50 µM mercaptoethanol
Subculture:
seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days, maintain at 0.5-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Gallus gallus species.
Viruses:
SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list