LLC-PK1
ACC 637
Cell line:
LLC-PK1
DSMZ no.:
ACC 637
Species:
porcine (pig) (Sus scrofa)
Cell type:
kidney
Origin:
established in 1958 from the normal kidney of a juvenile male Hampshire pig (Sus scrofa); cells were described in the literature to exhibit properties characteristic of kidney proximal tubular epithelium
Biosafety level:
1
Risk assessment:
The cell line carries Porcine type-C oncovirus (PCOV) sequences and their transcripts, which were detected by PCR and RT-PCR, respectively. We did not determine the infection mode of these viruses and cannot exclude the secretion of the viruses. In Germany, these viruses are classified BSL 1 regarding men and BSL 2 relating to animals (TRBA 462). However, the German Central Committee on Biological Safety (ZKBS) classified these viruses and infected cell lines BSL 2 when they are used for genetically modifying applications (Ref. 6790-10-65).
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; optimal split ratio of 1:5 to 1:10 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Sus scrofa species.
Viruses:
SMRV -
Supplied as: