BT-474
ACC 64
Cell line:
BT-474
DSMZ no.:
ACC 64
Species:
human (Homo sapiens)
Cell type:
breast ductal carcinoma
Origin:
established from the primary tumor of an invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus; described to carry a mutation in the TP53 gene, rendering cells temperature sensitive; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 10 µg/ml human insulin + 2 mM L-glutamine
Subculture:
seed out at ca. 1-3 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 7-10 days using trypsin/EDTA; cells grow slowly and may produce cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100 hours
Harvest:
about 1.0-1.6 x 105 cells/cm2 or 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent (MRA), thereafter negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertetraploid karyotype - 104(91-111)<4n>
Molec. Genetics:
expression of ER, PR, AR and HER2 determined by Western blot
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list