PK-15
ACC 640
Cell line:
PK-15
DSMZ no.:
ACC 640
Species:
porcine (pig) (Sus scrofa)
Cell type:
kidney
Origin:
derived by single-cell cloning of the parent cell line PK-2a in 1957, which was established in 1955 from the kidney of an adult pig; cells were shown to carry an endogenous C-type retrovirus and to be susceptible to various viruses, e.g. viruses causing Hog cholera, African swine fever, and foot and mouth disease
Biosafety level:
1
Risk assessment:
The cell line releases Porcine type-C oncoviruses (PCOV). In Germany, these viruses are classified BSL 1 regarding men and BSL 2 relating to animals (TRBA 462). However, the German Central Committee on Biological Safety (ZKBS) classified these viruses and infected cell lines BSL 2 when they are used for genetically modifying applications (Ref. 6790-10-65).
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently as monolayer
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:10 every 3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
maximum densitiy of ca. 8-10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Sus scrofa species.
Viruses:
SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list