RPMI-7951
ACC 66
Cell line:
RPMI-7951
DSMZ no.:
ACC 66
Species:
human (Homo sapiens)
Cell type:
malignant melanoma
Origin:
established from an involved lymph node of an 18-year-old woman with malignant melanoma in 1971
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:4 to 1:10 every 4-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60 hours
Harvest:
about 10-12 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 22% polyploidy - 54(50-60)<2n>X, -X, +1, +2, +6, +7, +8, +20, +20, +3mar, der(8)t(7;8)(p11;p12), der(9)t(9;11)(p21;q11), add(18)(q23;HSR) - shares t(9;11) with IPC-298 defining a new recurrent translocation in malignant melanoma
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list