BCBL-1

ACC 683

Cell line:

BCBL-1

DSMZ no.:

ACC 683

Species:

human (Homo sapiens)

Cell type:

B cell lymphoma

Origin:

established from the peritoneal effusion of a 40-year-old man with B-NHL (primary effusion lymphoma, PEL); cells were shown to carry the human herpesvirus type 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Reference(s):

15854, 14483, 15200, 18187

Biosafety level:

Risk assessment:

The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.

Permissions and restrictions:

A, B

DSMZ Cell Culture Data:

Morphology:

round/multiformed cells growing singly in suspension; image

Medium:

80% RPMI 1640 + 20% h.i. FBS

Subculture:

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca. 0.3-1.0 x 10⁶ cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; immediately after thawing, viability might be rather low (60-70%)

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 50 hours

Harvest:

maximum densitiy of ca. 1 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human highly rearranged flat-moded hypertetraploid karyotype with near-diploid and near-octaploid sidelines; 91-100<4n>XX, -Y, -Y, +2, +2, +6, +7, +7, +8, -9, -10, -11, +12, -13, -13, +15, -16, -22, -22, +2, -5mar, dup(1)(q12q44), del(1)(p11)dup(1)(q12q44), del(2)(p11)x1-2, der(2)t(2;5)(q35;?q32)t(5;8)(?q35;q22), der(4)t(4;8)(q24;q24), t(6;17)(p23;q21), del(9)(q12q21), der(11)t(11;15)(q23;q21), der(11)t(11;15)(q25;q25), der(13)t(13;13)(p12;q21)x1-2, der(14)t(4;14)(q31;q32)x2, der(14)t(14;22)(p12;q12)t(4;14)(q31;q22)x2, der(15)t(8;15)(q21;q25); carries IGH rearrangements and genomic MYC amplification; related to published karyotype

Viruses:

PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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