KASUMI-6

human (Homo sapiens)

acute myeloid leukemia

established in 1999 from peripheral blood of a 68-year-old man with acute myeloid leukemia (AML-M2) after relapse; cells are described in the literature to carry a dominant-negative CEBPA (C/EBPA) mutation

15974, 40397

1

A

round cells growing singly in suspension; image; image; image

80% RPMI 1640 + 20% h.i. FBS (optional + 10 ng/ml GM-CSF)

cells are difficult to culture; seed out at ca. 0.5 x 10⁶ cells/ml, maintain at 0.4-1.0 x 10⁶ cells/ml (do not exceed these limits), split saturated culture 1:2 every 4-5 days; upon thawing viability drop to about 50% and may need one week to recover; accumulation of calcium phosphate (hydroxyapatite) in the culture was described in ref Harasawa and observed by us - particles can be tiny (resembling bacterial infection) or form larger, dark aggregates with irregular surface; washing of cells with PBS is recommended to reduce the amount of debris; cells were described to be constitutively cytokine-dependent, however, we found that the cells are no more cytokine dependent.

at 37 °C with 5% CO₂

ca. 4-5 days

maximum density at ca. 1 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD4 -, CD13 +, CD14 -, CD19 -, CD33 +, CD34 -, cyCD68 -, HLA-DR +

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human flat-moded hyperdiploid karyotype; 47-51<2n>XY, +5, +8, +10, +13, +22, inv(5)(p11q32), add(6)(p22.1), add(8)(p23)x1-2, der(12;17)(q10;q10), der(13)t(13;13)(p12;q31), dup(19)(q11q13); resembles published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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