Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peripheral blood sample of a 48-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 2A, at relapse) in 1984; cells were described in the literature to be EBV-negative, to carry the t(8;14) translocation effecting MYC-IGH and a TP53 point mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml in flasks; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3-1:5 every 2 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-24 hours
Harvest:
maximum density at ca. 3.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 3% polyploidy; 46(42-48)<2n>XY, inv(6)(q12q21), t(8;14)(q24;q32), del(18)(q21.1); carries t(8;14) effecting MYC-IGH rearrangement; matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
