KI-JK
ACC 695
Cell line:
KI-JK
DSMZ no.:
ACC 695
Species:
human (Homo sapiens)
Cell type:
anaplastic large cell lymphoma
Origin:
established in 1989 from the pleural effusion of a 15-year-old boy with Ki-1 positive lymphoma (anaplastic large cell lymphoma; ALCL); carries the translocation t(2;5) leading to NPM1-ALK fusion gene; cells were originally described in the literature to be EBV positive; however, viral analyses (PCR and western blot) at the DSMZ established that the cells do not harbor EBV
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round to polymorph cells growing in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are difficult to culture; seed out at ca. 0.2-0.4 x 106 cells/ml (we recommend 12- or 24-well plates); cells grow slowly at first; viability may be low (60-70%) after thawing; later split culture 1:3 every 3-4 days and maintain at 0.1-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximum density at ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD4 -, CD14 -, CD19 -, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype;
75-82 <3n>XXYY, +1, +1, +2, +3, +6, +7, +8, -9, +16, -15, -17, +19, +20, +21, +2-3mar, add(Y)(q11), del(1)(p34)x2, der(1)t(1;5)(p11;q11)t(5;2)(q35;p21), der(1;16)(q10;p10), i(1)(q10)x2, t(2;5)(p23;q35)x1-2, add(4)(p15), add(9)(p22), der(15)add(15)(p11)dup(15)(q11q26)add(15)(q26), der(16)t(1;16)(q21;q13), der(19)t(7;19)(q12.2;q11), der(19)t(8;19)(p12;p13.1), add(21)(p11);
sdl with del(7)(p12), add(9)(p21);
carries t(2;5) effecting NPM1-ALK rearrangement
Molec. Genetics:
expression of fusion gene NPM1-ALK confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list