Species:
human (Homo sapiens)
Cell type:
acute megakaryoblasic leukemia
Origin:
established in 1989 from the bone marrow of a 66-year-old man with acute megakaryoblastic leukemia (AML M7). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polymorphic cells growing singly and in clumps in suspension, 1% giant cells;
image;
image;
image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; culture may contain a high amount of cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum density at ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near tetraploid karyotype with 5% polyploidy; 86-93<4n>XX, -Y, -Y, +1, -4, +5, +6, -9, +13, -16, -18, -18, +19, +21, -22, +1-3mar;
del(1)(q11), i(5)(p10)x2, del(6)(q21q27), add(7)(p11), dup(13)(q21q31), i(del)(13)(q11q31)x2, i(18)(q10), add(19)(p13), del(19)(q11)x1-2, add(21)(q21.2)x1-2, qdp(21)(q22.?1q22.?3)x2; resembles published karyotype; qdp(21) targets rearrangement and amplification of RUNX1 (AML1)
Molec. Genetics:
expression of fusion gene RBM6-CSF1R confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
