
FLO-1
ACC 698
Cell line:
FLO-1
DSMZ no.:
ACC 698
Species:
human (Homo sapiens)
Cell type:
esophageal adenocarcinoma
Origin:
established in 1991 from a primary tumor of a 68-year-old man with esophageal adenocarcinoma; described to carry a TP53 mutation; whole genome sequence data in ref 40877
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells in a 75 cm2 or 175 cm2 flask; split confluent culture 1:4 to 1:5 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype;
82-89<4n>XXY, +1, -2, +7, +8, +8, -10, -10, -11, +16, +16, -17, -18, -18, +19, -20, +4mar; add(1)(q11), i(10)(q10), add(19)(q13), add(19)(q13)x2, add(20)(q13)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list