ESO-26
ACC 708
Cell line:
ESO-26
DSMZ no.:
ACC 708
Species:
human (Homo sapiens)
Cell type:
esophageal adenocarcinoma
Origin:
established from the primary tumor (pT4N1M1) of a 56-year-old man with adenocarcinoma of the esophagus in 2000; cells are described in the literature to carry a p53 mutation (C742T); whole genome sequence data in ref 40877
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 2-5 x 106 cells/175 cm2 flask; split culture 1:2 to 1-6 every 2-6 days using trypsin/EDTA or possibly by tapping the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-50 hours
Harvest:
cell harvest of ca. 100 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype; 39(36-40)<2n>X, -Y, +2, -3, -5, -11, -12, -15, -16, -19, -20, -22, +mar
add(X)(p22), del(1)(p32), add(12)(p13), der(13;21)(q10;q10), i(13)(q10), add(17)(p12), add(?18)(q21), add(21)(p11)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list