NAMALWA.IPN/45
ACC 71
Cell line:
NAMALWA.IPN/45
DSMZ no.:
ACC 71
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the tumor mass of a boy with Burkitt lymphoma; this variant subline from the original NAMALWA (ACC 24) was described in the literature to be characterized by differential expression of immunoglobulin genes, MYC genes and cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. Additionally, this cell line carries sequences of the squirrel monkey retrovirus (SMRV). According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Harvest:
maximal density of about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
CD3 -, CD13 -, CD19 +, CD37 +, HLA-DR +, smIgM +, smIgG -
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype - 45(42-49)<2n>X, -Y, -3, +7, ins(1;?)(q11;?), ins(3) (q11;q27-29), der(5)t(5;?)(p15;?), der(12)t(12;?)(q24;?), der(15)t(15;?)(p11;?) - NAMALWA marker chromosomes present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list