MAVER-1

ACC 717

Cell line:

MAVER-1

DSMZ no.:

ACC 717

Species:

human (Homo sapiens)

Cell type:

B cell lymphoma

Origin:

established from the peripheral blood of a 82-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma) in 2003; described in the literature to carry the t(11;14) affecting the CCND1 (BCL1) and IGH genes

Reference(s):

16453

Biosafety level:

Permissions and restrictions:

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

round cells growing singly or in small clumps in suspension; image; image; image; image;

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/ml; maintain at ca. 0.5-1.5 x 10⁶ cells/ml; split culture 1:2 every 2-3 days; we recommend to start the culture with 20% FBS

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 48 hours

Harvest:

maximum density at ca. 2 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human highly rearranged extreme hypodiploid karyotype with 10% polyploidy; 40(34-42)<2n>XY, -8, -12, -13, -14, -17, -22, der(1)dup(1)(p34p36)t(1;3)(p36;q21), der(3)del(3)(p13)t(3;14)(q27;q12), der(4)t(4;8)(q35;q24), der(6)t(6;14)(q13;q11)t(14;11)(q32;q13), der(8)dup(8)(q22q24)ins(8;7)(q24;?), ider(9)(q10)t(8;9)(q24;q34-35)t(8;22)(q24;q11), ider(9)del(9)(p21), der(10)t(8;10)(q13;p12), der(11)t(11;14)(q13;q32), der(12)t(12;12)(p13;q24)?del(12)(p11q11), der(14)t(3;14)(q13;q12), der(15)t(11;15)(p13;p11)dup(15)(q11q25), der(21)t(13;21)(q31;p11), der(22)t(18;22)(q22q23)dup(18)(q22q23)t(18;11)(q23;p13); resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1 together with non-IGH rearrangements of MYC and amplification of IGH/CCND1 and BCL2 regions; karyotypically related but distinct sidelines present

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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