NCEB-1
ACC 721
Cell line:
NCEB-1
DSMZ no.:
ACC 721
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1987 by Epstein-Barr virus (EBV) transformation of neoplastic peripheral blood cells from a 57-year-old man with centroblastic-centrocytic diffuse B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma); cells stably carry 5-8 mouse chromosomes and a t(11;14) leading to alteration of CCND1 (BCL1) and IGH genes
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round to polymorphic cells growing singly or in clumps in suspension, some cells are adherent; image
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split culture 1:2 every 2-3 days; maintain at ca. 0.5-1.3 x 106 cells/ml; adherent cells can be detached by tapping the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgM +, cyIgG -, cykappa -, cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
unique predominantly human, human/mouse hybrid flat-moded hypotetraploid karyotype; 82-91<4n>XX, -Y, -Y, -8, -12, -13, -13, -15, -15, -17, +6 - 7 mouse chr., del(X)(q21), der(5)t(5;12)(p15;q13)x2, del(7)(p21)x2, der(9)t(X;9)(q25;q32)t(X;13)(q27;q21)x2, add(10)(q25)x1-2, der(11)t(11;14)(q13;q32), t(11;14)(q13;q32)x2; resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1; mouse chromosomes may have been acquired during early passage on mouse macrophage feeder layers
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list