OCI-LY1
ACC 722
Cell line:
OCI-LY1
DSMZ no.:
ACC 722
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the bone marrow of a 44-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; diffuse large cell), stage 4B at relapse in 1983; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2 x 106 cells/ml; split saturated culture 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +, cykappa +, cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 6% polyploidy; 47(41-48)<2n>XY, +7, der(4)t(4;7)(p14;q22), der(7)t(4;7)(p14;p14)del(7)(q22), der(8)t(8;13)(p13;q13), der(8)t(8;8)(p22;q11), der(10)t(10;14)(p14;q32)t(14;11)(q32;q24), der(11)t(11;14)(q24;q32)t(14;18)(q32;q21), del(13)(q31), t(14;18)(q32;q21), der(18)t(3;18)(q24;q21)t(18;18)(p12;q21);
carries t(14;18) rearrangements, some cryptic, effecting juxtaposition of IGH with BCL2;
resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list