OCI-AML6
ACC 730
Cell line:
OCI-AML6
DSMZ no.:
ACC 730
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1991 from the peripheral blood of a 68-year-old woman with myelodysplastic syndrome (MDS) that transformed into acute myeloid leukemia (AML FAB M4); cell growth is dependent on cytokines, like GM-CSF, G-CSF or SCF (some of which are present in the conditioned medium of the cell line 5637)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10-20 ng/ml GM-CSF)
Subculture:
seed out at ca. 0.7 x 106 cells/ml in a 24-well plate; maintain at ca. 0.8-1 x 106 cells/ml; optimal split ratio 1:2 every 4-5 days; adherent cells can be detached by tapping the flask; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-5 days
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD11b +, CD13 +, CD15 +, CD19 -, CD33 +, CD34 +, cyCD68 +, CD117 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 6% polyploidy; 42-45<2n>XX, -3, -7, -15, -18, +21, t(1;10)(q21;q22), der(3)t(3;12)(p26;q23.2)ins(12;3)(q24;q21q26), der(4)del(4)(q11q25)ins(4;20)(q11;q12q13), del(5)(q13), der(11)t(3;11)(q26;p12), der(12)inv(12)(p13q13)del(12)(q23), der(13)t(13;18)(p11;q11.2), del(16)(p13), der(21)t(11;21)(p15;p11)x2; previously unkaryotyped; t(3;11) effects rearrangement of MECOM
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list