MDA-MB-231
ACC 732
Cell line:
MDA-MB-231
DSMZ no.:
ACC 732
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established in 1973 from the pleural effusion of a 51-year-old woman with invasive breast carcinoma after chemotherapy; widely used and extensively characterized cell line; triple-negative and described to be tumorigenic in nude mice; listed in the CCLE (ref 18232); a derivative expressing Cherry Fluorescent Protein is available (ACC 982); RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Leibovitz´ L15 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/175 cm2; optimal split ratio 1:5 to 1:20 every 3-5 days using trypsin-EDTA
Incubation:
at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed)
Doubling time:
ca. 25-30 hours
Harvest:
cell harvest of ca. 10-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
triple-negative confirmed by absence of ER, PR, AR and HER2 in Western blots
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list