human (Homo sapiens)
established from the pleural effusion of a 43-year-old woman with breast carcinoma in 1970 after radiation and chemotherapy. Widely used and extensively characterized cell line; decribed as ER-negative, but HER2neu positive; listed in the CCLE (ref 18232)
Permissions and restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
80% McCoy´s 5a + 20% h.i. FBS
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA for 5-10 min
at 37 °C with 5% CO2
ca. 2-3 days
cell harvest of ca. 5 x 106 cells/80 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in microbiological culture, PCR assays
To inquire about expression of EpCAM and intermediate filaments, contact email@example.com.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, BPyV -
|Frozen culture||400,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||500,- €|
|DNA isolated from cell line (5 µg)||120,- €|
see price list