SK-BR-3
ACC 736
Cell line:
SK-BR-3
DSMZ no.:
ACC 736
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion of a 43-year-old woman with invasive breast adenocarcinoma in 1970 after radiation and chemotherapy. Widely used and extensively characterized cell line; decribed as ER-negative, but HER2neu positive; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% McCoy´s 5a + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA for 5-10 min
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-3 days
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
Western blot: ER, PR and AR negative but HER2 positive
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, BPyV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list