T-47D
ACC 739
Cell line:
T-47D
DSMZ no.:
ACC 739
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established from the pleural effusion of a 54-year-old woman with infiltrating ductal carcinoma of the breast in 1974; cells were described in the literature to highly express the Wnt-7b oncogene, and positive for the estrogen and progesterone receptor and to express low amounts of androgene and glucocorticoid receptor; RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
85% RPMI 1640 + 15% h.i. FBS + 10 µg/ml human insulin
Subculture:
seed out at ca. 2-4 x 106 cells/175cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of ER, PR, AR and HER2 confirmed by Western blot
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list