Leibniz Institute DSMZ: Details

Cell line:

MEL-JUSO

DSMZ no.:

ACC 74

Species:

human (Homo sapiens)

Cell type:

melanoma

Origin:

established from the primary tumor of a 58-year-old woman with melanoma in 1977

Reference(s):

Biosafety level:

1

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epithelial-like adherent cells growing in monolayers; image; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 1-2 x 10⁶ cells/80 cm²; split confluent culture 1:5 every 3-4 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 28 hours

Harvest:

about 10 x 10⁶ cells/80 cm² flask

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Molec. Genetics:

Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61L mutation (mu/wt), but no BRAF V600 mutation

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €