U-CH2
ACC 749
Cell line:
U-CH2
DSMZ no.:
ACC 749
Species:
human (Homo sapiens)
Cell type:
chordoma
Origin:
established in 2000 from the malignant sacral chordoma of a 72-year-old woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
typical physalipherous chordoma phenotype growing strictly adherent
Medium:
80% mixture of Iscove's MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine
Subculture:
seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 5-7 days
Harvest:
cell harvest of ca. 3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list