Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1973 from the inguinal tumor of an EBV-negative 5-year-old girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polymorphic cells growing in suspension singly cells and in clumps;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-0.7 x 106 cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
