SK-MM-1
ACC 758
Cell line:
SK-MM-1
DSMZ no.:
ACC 758
Species:
human (Homo sapiens)
Cell type:
plasma cell leukemia
Origin:
established in 1981 from the bone marrow of a 55-year-old man with plasma cell leukemia at relapse after chemotherapy; cells are described in the literature to carry the t(6;14) effecting rearrangement of the IRF4 (MUM1) and IGH genes and t(14;20) leading to juxtaposition of IGH to MAFB
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 1.8 - 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD21 -, CD30 +, CD34 -, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat moded hypodiploid karyotype; 41-45<2n>X, -Y, +1, -13, dic(1;13)(p13.2;p13), t(4;19)(q11;p11), del(6)(q25), der(6)t(6;14)(p25;q32), der(8)t(8;14)(q24;q13)inv(14)(q13q32)t(14;6)(q32;p25)x2, der(14)t(8;14)(q24;q32)t(14;20)(q32;q11), der(15)t(11;15)(q12;p11), der(20)t(14;20); resembles published karyotype; carries concurrent t(6;14) and t(14;20) respectively rearranging IGH with IRF4 (MUM1) and MAFB plus MYC
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list