human (Homo sapiens)
established in 1974 from the moderately well-differentiated primary colonic adenocarcinoma (Duke´s type B) of a 58-year-old woman; cells were described in the literature to produce large amounts of carcinoembryonic antigen (CEA) and to express C-Myc, N-Myc, H-Ras, and N-Ras
Permissions and restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
90% RPMI 1640 + 10% h.i. FBS
seed out at ca. 2-4 x 106 cells/80 cm2 flask; split confluent culture 1:5 - 1:10 every 3-5 days using trypsin/EDTA
at 37 °C with 5% CO2
ca. 30-40 hours
cell harvest of ca. 20-25 x 106 cells/80 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in PCR assay
To inquire about expression of EpCAM and intermediate filaments, contact email@example.com.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
|Frozen culture||450,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||560,- €|
|DNA isolated from cell line (5 µg)||140,- €|
see price list