Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1983 from the bone marrow sample of a 52-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 4B) at relapse; cells were described in the literature to be EBV-negative and lacking a classical t(8;14) translocation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.2-0.3 x 106 cells/ml, at best in a 12- or 24-well plate; after thawing cell viability may be as low as 50%, but cells should recover quickly within a week; later, maintain at ca. 0.1-0.8 x 106 cells/ml and
split 1:3 - 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
maximum density at ca. 0.9 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 (+), CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; in the referenced paper this cell line is described as cykappa +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype; 72-77<3n>XXYY, +1, +9, -10, +13, +14, -17, +19, +20, +22, der(1)t(1;17)(p13;q12)x2, der(4)t(4;18)(q31;q21)x2, del(6)(q13)x2, der(6)t(6;6)(p24;q12), der(7)t(6;7)(p24;p22), der(14)t(14;19)(q32;q13.3)x2, del(18)(q21), der(19)t(4;19)(q21;q13)t(4;18)(q31;q21)x2, der(19)t(14;19), dup(20)(q11q13)x2; sdl with der(6)t(6;12)(p21;q21), der(7)t(5;7)(?p15;p24) etc; resembles published karyotypes; carries cryptic t(14;19) with rearrangement of IGH and SPIB, and t(4;18) with copy number amplification of the BCL2 region
Viruses:
PCR: HBV -, HCV -, HIV-1 -, HIV-2 -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
