Species:
human (Homo sapiens)
Cell type:
hairy cell leukemia
Origin:
established in 1981 from the peripheral blood of an 86-year-old man with hairy cell leukemia. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. Furthermore, genetic analyses revealed that the cells contain a partial genome of EBV. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Morphology:
lymphoblastoid cells growing as single cells or in clumps in suspension;
image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD71 +, CD80 +, CD138 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cyIambda -
;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-tetraploid karyotype
84-96<4n>XXYY, +2, -6, +9, +14, -19, -19, der(X)t(X;21)(p22;q22), der(Y)t(Y;19)(p11.3;q11)x2, der(17)t(11;17)(q11;p11)x2, der(17)t(17;22)(p11;q11), der(18)t(16;18)(q11;p11.3)x2-3; Subclones with additional numerical and structural alterations: e.g. t(15;17)(q25;q23)der(17)t(17;22)(p11;q11)
resembles published karyotype
Molec. Genetics:
exome sequencing revealed a BRAF D594E mutation
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -; NGS: EBV +
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
