Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1988 from leukemic B cells of a man with chronic lymphocytic leukemia (CLL); cells are described in the literature to be in vivo EBV-infected. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
irregularly round cells in suspension, partly aggregating or slightly adherent
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out and maintain at ca. 0.5-1 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days; the culture usually contains much cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 -, CD5 -, CD10 -, CD13 -, CD14 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, CD138 -, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 6% polyploidy; 47(45-48)<2n>XY, +12, der(13)t(3;13)(q25;p11)dup(13)(q11q34); trisomy 12 associated with CLL; resembles published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
