CI
ACC 770
Cell line:
CI
DSMZ no.:
ACC 770
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1978 from Epstein-Barr virus (EBV) immortalized lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml; optimal split ratio 1:2 - 1:4 every 2-4 days; growth of cells is feeder-layer independent
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-60 hours
Harvest:
maximum density at ca. 4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 9% polyploidy; 46-50<2n>XX, +12, del(13)(q14q22); sdl with +3 with del(13); +12 and del(13) recurrent in CLL; resembles published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list