CII
ACC 773
Cell line:
CII
DSMZ no.:
ACC 773
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1979 from Epstein-Barr virus (EBV) immortalized neoplastic lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); the CI cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
semi-adherent cells show round or ovoid morphology growing in clusters; long cultivation period leads to attachment of the cells and epitheloid morphology; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/25 cm2; optimal split ratio 1:2 - 1:4 every 2-4 days; adherent cells can be detached by tapping (or trypsination)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
cell harvest of ca. 4 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype; 93-97<4n>XXXXX, +X, +12, der(1)t(1;20)(p36;p11), der(12)t(12;14)(p11;q11), add(17)(p11)x2, del(16)(q12); sdl with additional numerical and structural aberrations; some resemblance to published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list