232A4
ACC 774
Cell line:
232A4
DSMZ no.:
ACC 774
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
established from Epstein-Barr virus (EBV) immortalized normal B-cells of a man with chronic lymphocytic leukemia (CLL, Rai 0 stage at diagnosis)
Biosafety level:
2
Risk assessment:
The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round to polymorphic cells, growing singly or in clusters in suspension; the culture contains usually a large amount of cell debris
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; optimal split saturated culture 1:2 - 1:4 every 2-4 days; the culture usually contains a significant amount of cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximum density at ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype with 4% polyploidy; 46(43-46)<2n>XY, no consistent abnormality detected; matches published karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list