WSU-DLCL
ACC 779
Cell line:
WSU-DLCL
DSMZ no.:
ACC 779
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1988 from the malignant pleural fluid of a 32-year-old woman with diffuse large B cell lymphoma at terminal stage
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 3-4 days; cells start to proliferate after approximately one week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
maximum density at ca. 0.9 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, HLA-DR -, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list