HS-578T
ACC 781
Cell line:
HS-578T
DSMZ no.:
ACC 781
Species:
human (Homo sapiens)
Cell type:
breast carcinoma
Origin:
established in 1977 from the ductal carcinosarcoma of the breast of a 74-year-old woman; the cells were described in the literature to produce casein and to lack estrogen-receptor protein; RNA sequence data are available (see Ref 40906 and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
irregularely shaped epithelioid cells growing adherently in monolayers; image
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 - 1:4 every second day using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
triple-negative due to absence of ER, AR and PR and weak HER2 expression in Western blots
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list